Supporting data: Functional plasticity of the swim bladder as an acoustic organ for communication in a vocal fish

In this study, we show that the swim bladder of male plainfin midshipman fish (Porichthys notatus) exhibits reproductive state-dependent changes in morphology and function for sound production and reception. We quantified swim bladder morphometrics and utilized psuedolandmarking to identify differences in nonreproductive and reproductive male swim bladders. We utilized auditory evoked potential recordings, to measure the auditory sensitivity of saccular hair cells in response to sound pressure levels ranging from 97 - 154 dB re: 1 µPa to determine how the swim bladder contributes to midshipman auditory sensitivity. Finally, we used finite element modeling to explore the frequency response of nonreproductive and reproductive male swim bladders.
, Swim bladder morphometrics:
Nonreproductive and reproductive male plainfin midshipman were CT scanned and reconstructed using Slicer software. Following recontruction, swim bladder width, length, and horn length were calculated. Swim bladders were then pseudo-landmarked and compared using PCA analysis.
Auditory sensitivity:
Auditory evoked potentials were recorded from saccular hair cells in nonreproductive and reproductive male midshipman with swim bladders that were intact (i.e., control) or ablated (i.e., removed) using glass microelectrodes. Evoked potentials were recorded in response to pure tone stimuli (95 - 950 Hz) at sound pressure levels ranging from 97 - 154 dB re: 1 µPa. All experiments were performed in MATLAB.
Finite element analysis:
Models of nonreproductive and reproductive male swim bladders from 3D Slicer were imported into FreeCad and sectioned into 22.5 degree increments before  being exported to Strand7. Models were then meshed with triangular elements and given ..., , # 'Supporting data: Functional plasticity of the swim bladder as an acoustic organ for communication in a vocal fish'

1. Author Information
A. Principle Investigator
Joseph A. Sisneros
University of Washington
Seattle, WA 98195

B. Corresponding Author
Loranzie S. Rogers
Harvard University
Cambridge, MA 02138

2. Date of data collection: February 2022 - July 2022

3. Geographic location of data collection: Puget Sound and Hood Canal, WA, USA

4. Funding Sources
NSF-IOS-1933166 (J.A.S)
Lerner-Gray Memorial Fund of the American Museum of Natural History (N.R.L.)
NIH T32DC005361 (N.R.L.)
Bloedel Hearing fellowship (J.L.D.)
Fulbright fellowship and No. 0279-2021-0005 (121032300224-8) (Y.P.S.)
Howard Hughes Medical Institute Gilliam Fellowship grant no. GT15044 (L.S.R.)

SHARING/ACCESS INFORMATION

1. Licenses/restrictions placed on the data: CC0 1.0 Universal (CC0 1.0) Public Domain

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