Data for: Euglenozoan kleptoplasty illuminates the early evolution of photoendosymbiosis

Kleptoplasts (kP) are distinct among photosynthetic organelles in eukaryotes (i.e., plastids) because they are routinely sequestered from prey algal cells and function only temporarily in the new host cell. Therefore, the hosts of kleptoplasts benefit from photosynthesis without constitutive photoendosymbiosis. Here, we report that the euglenozoan
Rapaza viridis
has only kleptoplasts derived from a specific strain of green alga,
Tetraselmis
sp., but no canonical plastids like those found in its sister group, the Euglenophyceae.
R. viridis
showed a dynamic change in the accumulation of cytosolic polysaccharides in response to light–dark cycles, and
13
C isotopic labeling of ambient bicarbonate demonstrated that these polysaccharides originate in situ via photosynthesis; these data indicate that the kleptoplasts of
R. viridis
are functionally active. We also..., 1. Predicted proteins of R. viridis and Tetraselmis sp.
Quality control of the Illumina HiSeq reads of the R. viridis and Tetraselmis sp. transcriptomes was performed with the FastQC tool v0.11.6. Adapters, shortest reads (<36 bp), and poor-quality reads (mean Phred quality value of <15) were removed with the Trimmomatic tool v0.38. The transcriptomes were assembled with Trinity v2.0.6, and the proteins were predicted with TransDecoder v5.0.2 (https://github.com/TransDecoder/TransDecoder/releases/tag/TransDecoder-v5.0.2).
2. Amplification and sequencing of partial plastid 16S rDNA.
The total DNA was extracted from cells harvested from 16-day-old cultures of R. viridis and Tetraselmis sp., with the Wizard Genomic DNA Purification Kit (Promega, Madison, WI), according to the procedure provided by the manufacturer. To enhance the extraction efficiency, the Tetraselmis sp. cells were bead-treated before the extraction procedure. The partial 16S rDNA in the plastid was amplified with ..., Rapaza viridis peptides 
The file in fasta format contains 49482 peptides predicted by Trinity TransDecoder from the assembled Rapaza viridis transcriptome.
Rapaza_viridis_peptides.fas.zip
 
Tetraselmis sp. peptides

The file in fasta format contains 33774 peptides predicted by Trinity TransDecoder from the assembled Tetraselmis sp. transcriptome.
Tetraselmis_sp_peptides.fas.zip
 

16S rDNA alignment of R. viridis and Tetraselmis sp. 
Alignment of the partial plastid 16S rDNA from Rapaza viridis and Tetraselmis sp. (the prey of R. viridis). 
Plastid_16SrDNA.fas
 
Plastid genome sequence of Teraselmis sp. 
The complete plastid genome sequence of Tetraselmis sp. was obtained from the Rapaza virids culture. 
Tetraselmis_cp.fas
 
Movie of Rapaza viridis preying on Tetraselmis sp.
The movie starts immediately after adding the Tetraselmis cells to the R. viridis culture. Within minutes, R. viridis ingested the whole Tetraselmis cell by phagocytosis.
Rapaza_phagocytosis.mp4
 
Single-gene phyl...