Original datasets for Copper control of the suppressive capacity in regulatory T cells

To further explore the underlying mechanism by which Slc31a1 regulates the histone acetylation of suppressive genes, we performed mass-based targeted metabolomic analysis of expanded ChiKO vs. WT Tregs (Fig. S5D). Although we successfully harvested a considerable number of Tregs through ex vivo expansion, which met the sample requirements for metabolomics, long-term activation via high doses of IL-2 (2000 U/ml) and αCD3/αCD28 Dynabeads normalized the differences in energy metabolism between the KO and control groups, as shown in their metabolic profiles (Fig. S5D). Nevertheless, phosphoenolpyruvic acid (PEP) and cyclic AMP (cAMP) levels were greater and dCMP levels were lower in expanded ChiKO Tregs than in expanded WT Tregs (Fig. S5D). The higher PEP46 level may reflect an enhanced glycolytic state in expanded ChiKO vs. expanded WT Tregs.