Gut Microbiota and Metabolome Response of Decaisnea insignis Seed Oil on Metabolism Disorder Induced by Excess Alcohol Consumption

This study investigated the modulatory
effects of Decaisnea insignis seed
oil (DISO), which was rich
in palmitoleic acid (55.25%), palmitic acid (12.25%), and oleic acid
(28.74%), on alcohol-induced metabolism disorder in mice. Fifty mice
were orally administered with 38% alcohol (0.4 mL/day) and without
or with DISO (3, 6, and 12 g/kg) for consecutive 12 weeks. DISO inhibited
the alcohol-induced weight loss and liver function abnormality (p < 0.01) and shifted the profiles of cecal microbiome:
elevating the abundance of Lactobacillus, Ruminoccoceae_UCG_004 (p < 0.05) and decreasing abundance of Parabacteroides (p < 0.05). This treatment also regulated metabolome
response of amino acid and lipid metabolism in cecal content: upregulating
5-hydroxyindole-3-acetic acid (p < 0.05), 6-hydroxynicotinic
acid, 5-methoxytryptamine, nicotinamide, and nicotinic acid (p < 0.1) and downregulating androsterone, tryptophan,
and indole-3-acetamide (p < 0.05). DISO protected
against alcoholic liver injury and gut microbiota dysbiosis by enriching
the relative abundance of Lactobacillus, which was
positively associated with the improvement of intestinal permeability
and tryptophan metabolism.