GEP regulates stem cell marker expression in liver cancer cell line Hep3B.

GEP overexpression increased, while GEP suppression decreased the expression of β-catenin, Oct4, Nanog, Sox2 and ABCB5, but not CD133 and EpCAM.
Cells were dually stained for GEP and stem cell markers. Protein expression of GEP, β-catenin, Oct4, Nanog, Sox2 and ABCB5 was detected by intracellular staining, while that of CD133 and EpCAM was assessed by surface staining, and was then analyzed by flow cytometry. Data are expressed as mean percentage of positive cells ± SD from three independent experiments.
*P<0.05,
**P<0.01,
***P<0.001 when compared with Hep3B control.