CIL:40149, Mus musculus, CNS neuron (sensu Vertebrata). In Cell Image Library

Tissue came from either mice genetically engineered to overexpress human alpha synuclein (mouse strain, C57BL/6-DBA/2, overexpress a mutant form of alpha synuclein (A53T), or wild type animals from the same strain, anesthetized with nembutal, and perfused with 4% paraformaldehyde. Tissue was sectioned on a vibratome at a thickness of 80µm and immunostained with the following antibodies: mouse monoclonal anti-alpha-SYN; BD Transduction Laboratories, secondary conjugated to Alexa 488; rabbit polyclonal anti-mGluR5, Chemicon Ab. 5675, secondary conjugated to Rhodamine Red X. DAPI was dissolved in ProLong Mounting Medium and applied at the time of mounting. Section imaged using a Olympus Fluoview 1000, with a Olympus PlanApo 60X 1.4 NA objective.