In_Vivo_Transposon_Sequencing_Identifies_Gut_Colonization_Determinants

Data from transposon insertion sequencing using an inducible mutagenesis system, along with related microbial genome sequencing. A mariner transposase plasmid carrying a kanamycin resistance cassette under an arabinose-inducible promoter was transformed into wild-type strain (WT) or frameshift dinB mutant (dinBT). After arabinose induction and library preparation, approximately 10^7 mutants were used to orally infect C57BL/6 mice (n = 10). Colon tissue was collected three days post-infection; KanR mutants were recovered and sequenced. Comparison of input (LB medium) and output (colon) insertion frequencies identified genes essential for colon colonization.