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Tab A: Fig 3A. Relative expression of Gb3 and Gb4 mRNA in different trophoblast subtypes normalized to GAPDH mRNA. Fig 3C. Relative expression of Gb3 and Gb4 mRNA in hPTCCTB cells with and without Forskolin differentiation into STBs. Tab B: Fig 4A. B19V uptake in trophoblasts, UT7/Epo, and REH cells with or without trypsin treatment. Tab C: Fig 5A. Quantification of NS1 mRNA after B19V infection in different trophoblast subtypes and UT7/Epo cells. Fig 5B. Quantification of VP1/2 mRNA after B19V infection in different trophoblast subtypes and UT7/Epo cells. Tab D: Fig 6A. Transfection efficiency with a plasmid encoding green fluorescent protein (GFP), GFP positive cells were quantified as percentage of total cells. Fig 6B. NS1 mRNA expression in trophoblasts and UT7/Epo cells after B19V genome transfection. Fig 6C. VP1/2 mRNA expression in trophoblasts and UT7/Epo cells after B19V genome transfection. Tab E: Fig 7B. Quantification of cells with cytosolic distribution of dextrans in BeWo and JEG-3 cells in response to PEI treatment. Fig 7C. Quantification of NS1 mRNA levels at 24 h post-infection in BeWo cells treated or untreated with PEI. Fig 7E. Quantification of structural VP1/VP2 mRNA levels at 24 h and 48 h post-infection in BeWo cells treated or untreated with 5 µM PEI. Tab F: Fig 8A. Quantification of Epo mRNA levels in BeWo and JEG-3 cells under normoxia (Nx) and hypoxia (Hx). Fig 8C. Effect of exogenous Epo supplementation on proliferation and viability of UT7/Epo and BeWo cells. Fig 8D. NS1 and VP mRNA levels in BeWo cells transfected with full-length B19V genomes, with or without Epo supplementation. Fig 8E. Cell proliferation and viability of BeWo cells treated with 5 or 20 µM AG490. Fig 8F. NS1 and VP1/2 mRNA expression in BeWo cells treated with AG490 prior to B19V genome transfection. Tab G: S1A Fig. Relative expression of AXL mRNA in UT7/Epo and in different trophoblasts, normalized to GAPDH. Tab H: S2A Fig. Relative mRNA expression of Gb3 and Gb4 in BeWo cells treated or untreated with forskolin (FSK) normalized to GAPDH. S2B Fig. Relative mRNA expression of Gb3 and Gb4 in BeWo cells exposed to normoxia or hypoxia with or without Epo, normalized to GAPDH. Tab I: S4 Fig. Time-dependent increase in β-actin mRNA levels as a proxy for cell growth in trophoblasts, expressed as % of β-actin expression relative to day 0. (XLSX)




