Gene expression profiles of ALL cells

Using using ATLAS cDNA expression arrays, we performed gene expression profiling of primary leukemia cells from 31 infants and 30 non-infant children (pediatric patients) with ALL to determine if any of these signature genes are differentially expressed in infant ALL vs. pediatric ALL. Reduced expression levels of 6 of the 9 CD22deltaE12 signature genes that were represented on the human cDNA arrays, including TP53 and APC as well as MDM2, SATB1, CCNG1 and GNB2 discriminated infant BPL from non-infant BPL. Total RNA was extracted from primary ALL cells and human ATLAS c DNA expression arrays were used for gene expression profiling.