The numerical values underlying those figures in main text and supporting information are deposited in S1_Data.xlsx file.

The data include the following: (1) chemotactic responses of CNB-1Δ20/MCP2201 and CNB-1Δ20 in Fig 1B; (2) chemotactic responses of CNB-1Δ20/MCP2201 to citrate and malate using the transwell chemotaxis assay in Fig 1C; (3) chemotaxis of E. coli strain harboring MCP2201-Tar (2201-Tar) or not (WT E. coli) to malate on the gradient soft-agar plate in Fig 1E; (4) biofilm formation of P. aeruginosa strains harboring MCP2201-WspA (2201-WspA) or not (PA) in Fig 1F; (5) chemotactic responses to malate on the gradient soft-agar plates by CNB-1Δ20 cells harboring MCP2201 mutants in Fig 2C; (6) analytical ultracentrifugation assays of ligand-free, malate-bound (10 mM), and citrate-bound (10 mM) MCP2201-LBD in Fig 3A; (7) ITC assays of MCP2201-LBD oligomer dissociation in the absence and in the presence of citrate and malate in Fig 3B; (8) chemotactic responses to malate on the gradient soft-agar plate by CNB-1Δ20 cells harboring MCP2201 mutants in Fig 3E; (9) ITC assays of MCP2201-LBD mutants’ malate affinity in Fig B; (10) ITC assays of MCP2201-LBD mutants’ oligomer dissociation in the presence of malate in Fig C; (11) chemotactic responses to citrate on the gradient soft-agar plate by CNB-1Δ20 cells harboring MCP2201 mutants in Fig A. (XLSX)