Human endometrial transcriptome and progesterone receptor cistrome reveal important pathways and epithelial regulators

Context. Poor uterine receptivity is one major factor leading to pregnancy loss and infertility. Understanding the molecular events governing successful implantation is hence critical in combating infertility. Objective. To define PGR-regulated molecular mechanisms and epithelial roles in receptivity. Design. RNA-seq and PGR-ChIP-seq were conducted in parallel to identify PGR-regulated pathways during the WOI in endometrium of fertile women. Setting. Endometrial biopsies from the proliferative and mid-secretory phases were analyzed. Patients or Other Participants. Participants were fertile, reproductive aged (18-37) women with normal cycle length, without any history of dysmenorrhea, infertility, or irregular cycles. In total, 42 endometrial biopsies obtained from 42 women were analyzed in this study. Interventions. There were no interventions during this study. Main Outcome Measures. Here we measured the alterations in gene expression and PGR occupancy in the genome during the WOI, bas...