TABLE 2 in The Amidella clade in Europe (Basidiomycota: Amanitaceae): clarification of the contentious Amanita valens (E. - J. Gilbert) Bertault and the importance of taxon-specific PCR primers for identification

TABLE 2. — List of selected taxon-specific primers,showing the sequences,annealing temperatures (Ta), matching and positive control universal primers. ApoIf 2 is for Amanita ponderosa Malençon & R.Heim, the remainder are for A. pseudovalens comb. nov., stat. nov. a, White et al. 1990; b, Gardes & Bruns 1993; c, NLC2R is the reverse complement of NLC2 (Martin & Rygiewicz 2005); d, Hopple & Vilgalys 1999.
IDSequenceTa (°C)MatchingControlApoIf 25’ GAGTGTCATTCATATTCTC 3’50.3ITS4aITS3aApsIr 15’ TTGTTCATTAACAATTGTCTTTC 3’54.7ITS1FbITS4ApsIr 35’ GACACAAATTCATTTAGAAAG 3’51.5ITS3ITS4ApsLr 25’ AATGAATGGCCTGGCGA 3’59.1NLC2RcLR5d