These experiments were performed to show a serogroup conversion in Vibrio cholerae from O1 to O139. For this purpose, V. cholerae O1 WT = A1552 was grown on crab shell fragments to induce natural comp
tRNAs decoding standard 20 AA 104 Selenocysteine tRNAs (TCA) 0 Possible suppressor tRNAs (CTA,TTA,TCA) 0 tRNAs with undetermined or unknown isotypes 0 Predicted pseudogenes 0 Total tRNAs 104
Mutagenesis of Vibrio cholerae with TnphoA, followed by screening for fusions that were activated under low-iron conditions, led to the identification of seven independent fusion strains, each of whic