Data from: Molecular physiology of chemical defenses in a poison frog

Oophaga sylvatica transcriptomeTranscriptome from Oophaga sylvatica. Sequenced from skin, liver, and intestines from a single individual. Full description of sample processing and sequencing can be found in associated manuscript.Osylvatica_transcriptome_Catyetal.fastaOsylvatica reference proteomeOophaga sylvatica proteome generated from mRNA-based protein reference database for peptide matching, using the PHROG tool (Proteomic Reference with Heterogeneous RNA Omitting the Genome).Osylvatica_allPHROG_proteins.faGas Chromatography Mass Spectrometry for AlkaloidsGas chromatography/mass spectrometry data from the skin of the Oophaga sylvatica. Five frogs were caught in the rainforest in Ecuador and three were laboratory raised. Dorsal skin was isolated and alkaloids were extracted with methanol.AlkaloidsGCMS.zipOophaga sylvatica Plasma Proteomics (part 1 of 3)Tandem Mass Tag (TMT; Thermo Scientific, Waltham, MA, USA) labeling was performed according to manufacturer's instructions. TMT is a chemical label that allows the quantification of proteins from pooled samples by adding slight variations to the molecular mass of proteins. Full description available in the associated manuscript.plasmaproteomics.set1.tar.gzOophaga sylvatica Plasma Proteomics (part 2 of 3)Tandem Mass Tag (TMT; Thermo Scientific, Waltham, MA, USA) labeling was performed according to manufacturer's instructions. TMT is a chemical label that allows the quantification of proteins from pooled samples by adding slight variations to the molecular mass of proteins. Full description available in the associated manuscript.plasmaproteomics.set2.tar.gzOophaga sylvatica Plasma Proteomics (part 3 of 3)Tandem Mass Tag (TMT; Thermo Scientific, Waltham, MA, USA) labeling was performed according to manufacturer's instructions. TMT is a chemical label that allows the quantification of proteins from pooled samples by adding slight variations to the molecular mass of proteins. Full description available in the associated manuscript.plasmaproteomics.set3.tar.gzOophaga sylvatica Thermal Proteome ProfilingThis assay was performed to identify proteins that bind to alkaloids. The assay relies on thermodynamic principles of protein-ligand binding, where a protein that has bound its small molecule target has increased thermal stability, a shift detectable by tandem mass spectrometry. A single laboratory reared frog was used for this analysis.TPP.tar.gz