AltAnalyze functional predictions for confirmed CP differentiation AS events.

Splicing, protein, and miRNA binding site annotations are shown for alternative exons confirmed by RT-PCR. For each alternative exon, the corresponding gene name (Gene symbol), ANOVA AS differentiation pattern (ANOVA pattern: diff = common CP-NP differentiation, cardiac = CP-specific), splicing index (SI) fold change, relative AltAnalyze exon/intron position in the gene structure (Exon ID), gene-expression (GE) fold change (Δ) for the gene, AS annotations that correspond to the Exon ID, change in predicted protein length (length of the competitive protein isoforms in hESC->CP), and top corresponding domain/motif or miRNA binding site annotations (Primary functional Δ). Negative SI fold changes indicate increased alternative exon expression in CPs and vise versa. For primary function Δ annotations, gain indicates the increase in the expression of an alternative exon overlapping with that domain in CPs versus hESCs, a loss indicates a relative decrease in expression and a gain/loss indicates that the domain/motif is present in both protein isoforms but with different sequence. PS = phosphoserine modified residue, PT = phosphotyrosine modified residue, miRNA = miRNA binding site. Complete annotations can be found in Dataset S2.