Metabolic flux to ProFAR is required for PurF-independent PRA synthesis.

Growth rates (in hours−1) are shown. Strains were grown in minimal glucose medium with adenine and the indicated additions. His: histidine; Thi: thiamine. Histidine alleles hisA3000, hisI99, hisF109, his-2652 (del:CBHAFI) cause a complete loss of function of the relevant gene product(s). Allele hisG1102 encodes an enzyme that is insensitive to feedback inhibition by histidine.aNG = no growth; growth rate was −1.