Gene expression analysis following 24h- and 48h-siRNA knockdown of transforming growth factor β receptor III (TGFBR3) in human pulmonary arterial smooth muscle cells (hPASMC).. Homo sapiens

We explored the impact of siRNA-mediated knockdown of the transforming growth factor β receptor III (TGFBR3) on the gene expression in the human pulmonary arterial smooth muscle cells (hPASMC), at 24h and 48h. Overall design: Human pulmonary arterial smooth muscle cells (hPASMC) in P5 were plated in 6-well plates and transfected with either 200nM siRNA to transforming growth factor β receptor III (TGFBR3) (Santa Cruz Biotechnology) or Scrambled control (Ambion). Total RNA was extracted at 24 and 48h timepoints with peqGOLD TOTAL RNA Kit (VWR).