TABLE 1 in Introducing Nigrograna italica sp. nov. (Nigrogranaceae) from Corylus avellana and Valsaria tectonae sp. nov. (Valsariaceae) from Tectona grandis

TABLE 1. PCR amplification conditions for each locus used in this study.
LocusPrimers (forward/ reverse)Initial DenaturationDenaturationAnnealingExtensionReferencesSSUNS1/NS494 °C 3 mins94 °C 30s54 °C 50s72 °C 1.30 minsWhite et al. (1990)LSULR0R/LR595 °C 5 mins95 °C 45s53 °C 45s72 °C 2 minsVilgalys & Hester (1990)ITSITS5/ITS495 °C 5 mins95 °C 45s53 °C 45s72 °C 2 minsWhite et al. (1990)rpb2fRPB2-5f /fRPB2-7cR95 °C 5 mins95 °C 1 min54 °C 2 mins72 °C 1.30 minsLiu et al. (1999) Sung et al. (2007)tef1728F/2218R94 °C 5 mins94 °C 30s58 °C 1.30 mins72 °C 1.20 minsCarbone & Kohn (1999) Jaklitsch & Voglmayr (2016)