Menopausal Transition: Prospective Study of Estrogen Status, Circulating MicroRNAs, and Biomarkers of Bone Metabolism

We carried out Massive Parallel Sequencing (MPS) to reveal circulating miRNA profiles of 44 patients before and after oophorectomy, as well as in oophorectomized women on estrogen therapy. The sequencing platform identified 1305 miRNA species, out of which 439 miRNAs were present with the base mean, computed by DESeq2, more than 10. We identified 17 miRNAs, which were different in sera with sufficient and low estrogen levels. Overall design: miRNA profiling of 44 sera from women before and after oophorectomy, as well as in oophorectomized.