Gene expression of human colon cancer HCT116 cells [HOXA5_OE]

Abberant expression of HOXA5 is implicated in cancer development. We identified a novel long non-coding RNA (HOXA5 short RNA) transcribed from HOXA6-HOX5 locus using a next generation-based target RNA capture system. In this study, HCT116 cells were overexpressed with a vector containing HOXA5 short RNA. Gene expression levels of these cells were measured by microarray analysis. HCT116 cells were transfected with a pEB vector containg a novel long non-coding RNA (HOXA5 short RNA), and then, the cells were cultured in selection medium (blastcidin) for 2 weeks. Total RNA was extracted from these cells, and gene expression profiles were measured by human whole-genome microarray (Agilent Technology).