Cellular and transcriptional signatures unveiled by single cell RNA-Seq (scRNA-Seq) following ex vivo infection of murine splenocytes with Borrelia burgdorferi

Splenocytes comprised a wide-range of immune cells responsible for the protection against infectious diseases. We exposed splenocytes to GFP+ Borrelia, sorted GFP+ and GFP- splenocytes and performed single-cell RNA seq to analyze the DEGs between infected and bystander population in different immune cell populations We used single-cell RNA-Seq to study the splenocytes from C3H/HeN mice exposed to GFP-tagged Borrelia, sorted GFP+ splenocytes and GFP- bystander cells to uncover novel molecular and cellular signatures that contribute to early stages of immune responses against Bb.