Data and Code for: Phospholipase-catalyzed Degradation Drives Domain Morphology and Rheology Transitions in Model Lung Surfactant Monolayers

Lung surfactant is inactivated in acute respiratory distress syndrome (ARDS) by a mechanism that remains unclear. Phospholipase (PLA2) plays an essential role in the normal lipid recycling processes, but is present in elevated levels in ARDS, suggesting it plays a role in ARDS pathophysiology. PLA2 hydrolyzes lipids such as DPPC—the primary component of lung surfactant—into palmitic acid (PA) and lyso-PC (LPC). Because PA co-crystallizes with DPPC to form rigid, elastic domains, we hypothesize that PLA2-catalyzed degradation establishes a stiff, heterogeneous rheology in the monolayer, and suggests a potential mechanical role in disrupting lung surfactant function during ARDS. Here we study the morphological and rheological changes of DPPC monolayers as they are degraded by PLA2 using interfacial microbutton microrheometry coupled with fluorescence microscopy.  While degrading, domain morphology passes through qualitatively distinct transitions:  compactification, coarsening, solidifica..., , , # Data and Code for: Phospholipase-catalyzed degradation drives domain morphology and rheology transitions in model lung surfactant monolayers

[https://doi.org/10.5061/dryad.70rxwdc6k](https://doi.org/10.5061/dryad.70rxwdc6k)

## Description of the data and file structure

The data collected for the associated article includes both interfacial rheology collected using the microbutton microrheometry technique and micrographs collected using fluorescence microscopy. The interfacial rheology and micrograph data describe monolayers of the phospholipid DPPC being actively degraded by the enzyme PLA2.

The files included are:

* \"Figure Data for Dryad\" which corresponds to all plotted data in the associated article
* Folders for Figure 4, 5, 6, 7, 8 images include the full fluorescence micrographs corresponding to each figure in the associated article
* \"Degrading monolayer video example\" contains a sequence of sped up videos from a single monolayer being actively degraded by PLA2
* \"Sample ...