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Next Generation Sequencing of miRNA in PRRSV-infected PAMs

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NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE164993
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We used the high-throughput sequencing and inhibitors to screen microRNAs that play the role in anti-porcine reproductive and respiratory syndrome virus (PRRSV) responses in porcine alveolar macrophages (PAMs). Next Generation Sequencing of miRNA in PRRSV-infected PAMs of pigs. The miRNA library construction and sequencing were conducted with Illumina sequencing technology (BGI-Wuhan, China), and qualified sequence data were analyzed by GBI (Wuhan, China). In brief, only miRNAs represented by > 20 reads were selected for differential expression testing.
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2021-01-26
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