Proteins identified in more than one active, but not in the inactive CVL samples by LC-ESI-MS/MS.

Proteomic analysis was performed on the starting material (A), flow through (B), and retentate (C) after fractionation with a Centricon 50 kDa spin column from an active CVL pool (1) or two different individual CVL (2, 3). Sample 4 was separated on a gel filtration column and the starting material (A) and the fraction with the greatest bactericidal activity (D) were evaluated.