Single cell analysis of epithelial (EPI), immune (IMM) and stromal cells (double negative, DN) cells from murine small intestine in steady state following LXR activation.

LXRs (LXRa and LXRb) are widely expresded in a variety of cell types. We addressed how transient LXR activation using GW3965 impacts the cellular composition and/or transcriptional changes in the SI of WT mice. Overall design: WT mice were fed with either control (standard) or GW3965 containing diet for a period of 10 days. Following this, epithelial (EpCAM+CD45-, EPI), immune (EpCAM-CD45+, IMM) or stromal cells (EpCAM-CD45-, double negative, DN) cells were sorted and processed for single cell transcriptomics analysis using protocol from 10X genomics.