Chemical-based external stimulation reprograms human somatic cells into pluripotency (single cell RNA-seq)

Here, we report that chemical-based external stimulation reprograms human somatic cells to generate human induced pluripotent stem cells. Using transcriptome, chromatin accessibility and DNA methylome profiling, we identified the cells exhibited key features of embryonic stem cells in transcriptomic profiles, epigenetic status. In addition, we also analyzed the reprogramming process. Overall design: To dissect the molecular mechanism, the reprogramming process were analyzed by single cell RNA-seq.