Targeted PCR Rickettsia screen
收藏NIAID Data Ecosystem2026-03-11 收录
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https://figshare.com/articles/dataset/Targeted_PCR_Rickettsia_screen/12801206
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资源简介：
A targeted PCR
Rickettsia screen of 1,612 individuals from 169 species was
undertaken to increase our
understanding of Rickettsia ecology. Within this, we included a range of
both aquatic and terrestrial taxa, to investigate if the previous work highlighting
particular aquatic taxa as hotspots for Rickettsia symbiosis (water
beetles, biting midges, damselflies) reflects a wider higher incidence in
species from this habitat.
mtDNA COI amplification was conducted as a control
for DNA quality. To investigate symbiont infection status, rickettsial-specific
primers based on gltA and 16S rRNA genes were used for
conventional PCR screening, with Sanger sequences
obtained from at least one specimen per Rickettsia positive species to
identify any misamplification false positives.
创建时间：
2020-08-13
