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Mus musculus [C57BL/6 x Japanese fancy 1] F1 genome sequencing project. Mus musculus strain:[C57BL/6 x Japanese fancy 1] F1

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NIAID Data Ecosystem2026-03-07 收录
下载链接:
https://www.ncbi.nlm.nih.gov/bioproject/PRJDA72519
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资源简介:
C57BL/6 (B6) females were mated with Japanese fancy 1 (JF1) males to generate [B6xJF1]F1 mice and reciprocally crossed to generate [JF1xB6]F1 mice. Total RNA was extracted using ISOGEN (Nippon gene, Tokyo, Japan) and further purified using RNeasy mini Kit and RNase-free DNase (Qiagen, CA, USA) from four [B6xJF1]F1 and four [JF1xB6]F1 placentas at E13.5, respectively and pooled. Twelve micrograms of total RNA was used for rRNA depletion (RiboMinus Eukaryote kit for RNA-seq, Invitrogen, CA, USA) and RNA-seq library construction (SOLiD Whole Transcriptome Analysis Kit, Life technologies, CA, USA) according to the manufacturer's protocol.
创建时间:
2011-08-25
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