RNA-seq of primary islets in HFD_DMSO,HFD_CGP and ND mice

We investigated the underlying molecular mechanism of CGP 57380's effects in type 2 diabetes by comparing gene expression of whole primary islets isolated from HFD_DMSO,HFD_CGP and ND mice by RNA sequencing.C57BL/6 mice were fed an HFD for 4 months and then oral gavage DMSO and CGP 57380 for about 1 month separately to studied gene-expression differences resulting in HFD-induced hyperinsulinemia and metabolic abnormities.At the same time ,explore the therapeutic effect of CGP 57380 on type 2 diabetes,and the changes in the signal pathways in it.Gene set-enrichment ananlysis was performed using Kyoto Encyclopedia of Genomes algorithms and MSigDB software.The most enriched pathways were linked to pathyways associated with PI3K-Akt signaling pathway, pancreatic secretion and NF-kappa B signaling pathway. Overall design: Islets mRNA of HFD_DMSO,HFD_CGP and ND male mice