Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 5' terminal phosphates and crosslinked with EDC.

Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 5' terminal phosphates and crosslinked with EDC. Descriptor: DNA (5'-D(A*CP*CP*CP*GP*GP*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*AP*CP*GP*G)-3')|, DNA (5'-D(A*CP*CP*GP*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*GP*GP*G)-3'), MAGNESIUM ION, ... Authors: Orun, A.R, Shields, E.T, Shrestha, R, Slaughter, C.K, Snow, C.D. Deposit date: 2023-07-20 Release date: 2023-08-02 Last modified: 2024-11-06 Method: X-RAY DIFFRACTION (3.35 Å) Cite: Tuning Chemical DNA Ligation within DNA Crystals and Protein-DNA Cocrystals. Acs Nanosci Au, 4, 2024

本数据集为复制起始蛋白REPE54（Replication Initiator Protein REPE54）与对称扩展双链体（31聚体）的同网状互穿共晶体；该双链体包含同源REPE54序列与一段额外的富含G-C的序列，末端为平末端，带有5'端磷酸基团，且经EDC（1-(3-二甲氨基丙基)-3-乙基碳二亚胺）交联。描述符：DNA（5'-D(A*CP*CP*CP*GP*GP*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*AP*CP*GP*G)-3'）、DNA（5'-D(A*CP*CP*GP*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*GP*GP*G)-3'）、镁离子（MAGNESIUM ION）等。作者：Orun, A.R、Shields, E.T、Shrestha, R、Slaughter, C.K、Snow, C.D。入库日期：2023-07-20，发布日期：2023-08-02，最后修改日期：2024-11-06。实验方法：X射线衍射（3.35 Å）。引用：《Tuning Chemical DNA Ligation within DNA Crystals and Protein-DNA Cocrystals》，ACS Nanosci Au，第4卷，2024年。