The influence of upper interface single-, double- and triple-mutations on slow inactivation gating of the tandem dimer Shaker Kv channela

KI) and the free energy for inactivation gate closure (ΔGinactivation), for all single-, double- and triple-mutants used to calculate the coupling free energies during inactivation gating.a The table displays the forward and backward time constants for slow inactivation gating (at 0 mV) along with the inactivation equilibrium constant (KI were calculated according to KI = kf/kr (see Materials and Methods).b Values for ΔGinactivation were calculated according to ΔGinactivation = −RTln KI.c Values for Materials and Methods).d Values for the slow recovery phase are presented (see ΔΔGinactivation were calculated according to ΔΔGinactivation = (−RTln (kf/kb)wt −−RTln (kf/kb)m).e Values for