Mina53 regulates the expression of downstream genes in NSPC cells by its arginine demethylase activity on H4R3me2a

In order to investigate the role of Mina53 in the NSPC proliferation and differentiation, we performed RNA-seq using Mina53-KO NSPCs and wild-type NSPCs; we performed CUT-TAG using anti-H4R3me2a antibody in Mina53-KO NSPCs and wild-type NSPCs. NSPCs were extracted from relative mice and then purified. Then these cells were suspended using trizole. NSPCs were extracted from relative mice and then purified. Then these cells were performed CUT-TAG assay using NovoNGS® CUT&Tag 4.0 High-Sensitivity Kit (for Illumina®). Please note that the records have been updated with the CUT-TAG samples on Dec 13, 2024.