RNA-seq data of Zea mays subsp. mays under waterlogging stress. Zea mays subsp. mays

An inbred line HKI1105, tolerant to waterlogging stress, was chosen for the experiment. Two sets of disposable plastic cups, with five in each, (250 cm cups perforated at the base at four points with an orifice of ~5.0 mm diameter) were filled with pot mixture up to 220 cm of its volume. Seeds were sown in the cups by applying optimal water and the seedlings were allowed to grow till three leaf stage (27°C/24°C day/night and 16 h/8 h light/dark). After the three leaf stage, one of the sets was kept in a plastic tray (60  30  15 cm3) and water was added into the plastic tray above the level of plastic cups to induce the waterlogging stress to the seedlings. The second set was maintained as a non-stress (control) under optimal water condition. Five days after the stress (Zaidi et al., 2003) root tissues of stressed and non-stress plants were isolated and immediately frozen in liquid nitrogen.