Profiling of CD14+ monocytes from humans with Type diabetes and without diabetes [CHIRP-seq]

We identified a novel lncRNA DRAIR that is downregulated in CD14+ monocytes from type 2 diabetes relative to controls. Functional studies showed that DRAIR regulates anti-inflammatory genes and its knockdown enhances proinflammatoory phentype of monocytes. To examine mechanisms of DRAIR actions, we performed Chromatin isolation by RNA purification (ChIRP) assays using DRAIR biotinylated tiling oligonucleotides to identify chromatin inding sites in THP-1 monocytes. ChIRP-seq analysis of lncRNA DRAIR was performed in THP-1 monocytes to identify chromatin binding sites using published protocols (Chu et al, Molecular cell. 2011;44(4):667-78).