Effects on gene expression of doxorubicin in human stem cells-derived cardiomyocytes

To gain into the molecular mechanisms and pathways involved in doxorubicin induced cardiotoxicity, we performed whole genome transcriptome profiling via RNA-seq in hESCs-derived CMs treated with doxorubicin or vehicle only control. Cells were treated with 0, 1 or 2.5 µM of doxorubicin for 16 hours and RNA was isolated for high throughput sequencing Overall design: To identify genes that are significantly regulated by doxorubicin treatment, we analyzed read count data from RNA-seq using edgeR software. We also performed KEGG pathway analysis and GO analysis