Rare Earth Element Biomining from the Great Salt Lake Brine Using Engineered E. Coli
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This data describes rare earth element adsorption onto E. coli cells engineered to express a lanthanide binding tag (LBT). We used a Great Salt Lake synthetic solution as the background matrix with Tb added to 1-10,000 ppb, concentrations much lower than the competing ions present. Our results showed that Tb binds to LBT, even in the presence of high concentrations of competing metals. We also tested REE adsorption at elevated temperatures (up to 100 degrees Celsius), and observed that Tb adsorption increases with temperature of to 70 degrees Celsius, and then remains constant until 100 degrees Celsius. Data analyses were performed using an ICP-MS at UCSC.
本数据集描述了稀土元素在经过改造以表达镧系元素结合标签(LBT)的大肠杆菌细胞上的吸附现象。实验采用大盐湖合成溶液作为背景基质,其中添加了Tb,浓度范围为1-10,000 ppb,远低于存在的竞争离子浓度。研究结果显示,即使在竞争金属的高浓度存在下,Tb依然能够与LBT结合。此外,我们还对稀土元素在升高温度条件下的吸附进行了测试(高达100摄氏度),观察到Tb的吸附随着温度升至70摄氏度而增加,并在100摄氏度时保持恒定。数据分析采用加州大学圣克鲁兹分校的ICP-MS进行。
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