IGF-1-mediated microRNA profile in glioblastoma

Insulin-like growth factor (IGF)-1 signaling and micro (mi)RNAs are relevant in mediating GBM progression. However, their roles in desensitizing GBM cells to TMZ are still unclear. We aimed to identify IGF-1-mediated miRNA regulatory networks that elicit TMZ insensitivity for GBM. By miRNA array analyses, totally 1,308 miRNAs were examined. The 93 upregulated and 148 downregulated miRNAs were identified in IGF-1-treated glioma cells. Establishment of microRNA profile between IGF-1-treated and untreated GBM U87-MG cells. U87-MG cells were treated with or without 200 ng/ml IGF-1 for 48 h. Total RNA was extracted for microRNA array analyses.