Effect of knockout of DNAJC12 on gene expression in SH-SY5Y cells [RNA-seq]

We here reported DNAJC12 levels are negatively correlated with the prognosis of children with neuroblastoma and the knockout of DNAJC12 improves cell proliferation and invasion in neuroblastoma cells.DNAJC12 knockout results in increase of intracellular lactic acid and H4K5la modification level. DNAJC12 affects COL1A1 gene transcription by regulating H4K5la modification level and plays an important role in neuroblastoma metastasis. Overall design: To investigate the function of DNAJC12 in regulating SH-SY5Y cell invasion, we established SH-SY5Y cell lines in which DNAJC12 gene has been knockout via Crispr/cas9