The qPCR results of Asian arowana eDNA detection in Muda Lake, Kedah, Malaysia

We present a qPCR-based eDNA method to detect the presence of the endangered Asian arowana (Teleostei: Osteoglossidae: Scleropages formosus) in Muda Lake, where resides one of its last wild populations on the west side of Peninsular Malaysia. Flanked by a pair of newly developed species-specific primer, a 163-base pair fragment of the cytochrome oxidase subunit I (COI) gene was selected as the eDNA marker. We applied our eDNA method at five sampling sites throughout Muda Lake and at five different periods. The study observed a marked seasonal variation in detection rates, with significantly higher rates during the wet months of August, January, and December compared to the dry season spanning May and June. The Asian arowana is known to breed during the rainy season and we therefore hypothesize its reproductive biology influences the concentration of eDNA in the lake. Our results also highlight the possible impact of abiotic factors in tropical freshwater ecosystems (such as high-water t..., This dataset was obtained by conducting a qPCR amplification on environmental DNA (eDNA) samples collected from Muda Lake, Kedah in Peninsular Malaysia., , # The qPCR results of Asian arowana eDNA detection in Muda Lake, Kedah, Malaysia

[https://doi.org/10.5061/dryad.931zcrjs2](https://doi.org/10.5061/dryad.931zcrjs2)

## Description of the data and file structure

The dataset contents are to show detailed results of environmental DNA (eDNA) detection from several tests performed in a current study to detect the presence of Asian arowana in the lacustrine area in Peninsular Malaysia. The dataset contains detailed qPCR results of eDNA samples collected from aquaria, ponds, and a lake in a tropical environment of Peninsular Malaysia. The results are representing the Cq and melt peak values of each qPCR replicate from each water sample replicate. A detailed explanation of each eDNA detection was provided in the main document of the research article.