Construction the larval and adult zebrafish cell atlas at single cell resolution

Single-cell mRNA sequencing (scRNA-seq) technologies are reshaping the current cell-type classification system. In previous studies, we built the mouse cell atlas (MCA) and human cell landscape (HCL) to catalog all cell types by collecting scRNA-seq data. However, systematically study for zebrafish (Danio rerio) and fruit fly (Drosophila melanogaster) are still lacking. Here, we construct the zebrafish and Drosophila cell atlas with Microwell-seq protocols, which provides valuable resources for characterization of diverse cell populations of zebrafish and Drosophila, and studying difference between vertebrates and Invertebrates at single cell level. Overall design: Over 550,000 cells derived from zebrafish whole-body single-cell dissociation and profiling at the larval stage (72 hpf, 3 replicates, 10 fish for each replicate) and adult (90 day, 6 replicates, 1 fish for each replicate).