Highly sensitive amplicon-based transcript quantification by semi-conductor sequencing
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=f4f26edceba2921c53838b00158742dd
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资源简介：
We have adapted a commercial assay that employs mRNA quantification based on the frequency of PCR amplicons determined by next-generation to a high-throughput semi-conductor sequencing platform (Ion-Torrent Proton). We show parallel amplification of pathway derived transcript sets/genes in 12 reference RNA samples followed by sequence-based quantification covering a dynamic range of five orders of magnitude with low technical variation.
提供机构：
F. Hoffmann-La Roche
创建时间：
2022-02-20
