Ribosomal RNA 2’-O-methylation profile of human articular chondrocytes exposed to an osteoarthritic environment. Ribosomal RNA 2’-O-methylation profile of human articular chondrocytes exposed to an osteoarthritic environment

We used chondrocytes exposed to osteoarthritic synovial fluid as a chronic disease model to study the effects of a chronic disease microenvironment on 2’-O-me rRNA heterogeneity. Human non-OA human articular chondrocytes (5 individual donors) were cultured either in the presence of OA-SF (20% (v/v), pool of 14 donors) or in a normal culture medium and were refreshed every other day. After 14 days of culture, RNA was isolated for 2’-O-methylation profiling of ribosomal RNAs.