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<b>RAW DATA for "Protein Biosynthesis Is Associated with Ionome Composition in Soybean Seeds", by Montanha et al (2026)</b>

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NIAID Data Ecosystem2026-05-10 收录
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RAW DATA for "Protein Biosynthesis Is Associated with Ionome Composition in Soybean Seeds", by Montanha et al (2026)------------------------------------------------------------------------------------------------------------------------------------------------AUTHORS:Gabriel Sgarbiero Montanha (a,b,†)Lucas Coan Perez (b)Andrea Lepri (a)Chiara Longo (a)Davide Marzi (c,d)Eduardo Santos (b)Felipe Sousa Franco (b)Gustavo Paparotto Lopes (b)Nicolas Gustavo da Cruz da Silva (b)João Paulo Rodrigues Marques (e)Eduardo de Almeida (b)Alex Virgilio (f)Carlos Alberto Pérez (g)Paola Vittorioso (a)Sabrina Sabatini (a)Francisco Scaglia Linhares (h)Hudson Wallace Pereira de Carvalho (a)------------------------------------------------------------------------------------------------------------------------------------------------AFFILIATIONS:(a) Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, 00185 Rome, Italy(b) Group of Specialty Fertilisers and Plant Nutrition, Laboratory of Nuclear Instrumentation, Centre for Nuclear Energy in Agriculture, University of São Paulo, 13400-970 Piracicaba, Brazil(c) Research Institute on Terrestrial Ecosystems (IRET), National Research Council of Italy (CNR), Via Salaria km 29.300, 00015 Monterotondo Scalo (Rome), Italy(d) National Biodiversity Future Center (NBFC), 90133 Palermo, Italy(e) Laboratory of Applied Botany, Department of Basic Sciences, Faculty of Animal Science and Food Engineering, University of São Paulo, 13635-900 Pirassununga, Brazil(f) Laboratory of Analytical Chemistry, Centre for Nuclear Energy in Agriculture, University of São Paulo, 13400-970 Piracicaba, Brazil(g) Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Centre for Research in Energy and Materials (CNPEM), Rua Giuseppe Máximo Scolfaro, 10000, 13083-1000 Campinas, Brazil(h) Laboratory of Plant Developmental Biology, Centre for Nuclear Energy in Agriculture, University of São Paulo, Avenida Centenário, 303, 13400-970 Piracicaba, Brazil† Current affiliation: European Synchrotron Radiation Facility (ESRF), 71 Av. des Martyrs, 38000 Grenoble, France.------------------------------------------------------------------------------------------------------------------------------------------------DESCRIPTION:This dataset contains raw data supporting the manuscript. It includes:- Plant biometric measurements- Seed elemental concentrations- Total protein concentration data- SDS–PAGE densitometric analysis- 2D FTIR chemical imaging- Synchrotron-based XRF imaging- Light microscopy measurements- Micro-computed tomography (micro-CT) measurementsAll files are organised according to the corresponding figures presented in the manuscript.------------------------------------------------------------------------------------------------------------------------------------------------DATASET ORGANISATION:/Figure_1Data for Plant biometricsFile:Montanha-et-al_RawData_Fig-1.csvDescription: Plant biometric measurements used in Figure 1.------------------------------------------------------------------------------------------------------------------------------------------------/Figure_2Data for Seed elemental composition and protein contentFiles:Montanha-et-al_RawData_Fig-2_ICP-OES.csvDescription: Seed elemental concentrations determined by ICP-OES.Montanha-et-al_RawData_Fig-2_Protein-Dumas.csvDescription: Total seed protein content determined using the Dumas combustion method.------------------------------------------------------------------------------------------------------------------------------------------------/Figure_5Data for SDS–PAGE protein profilingFolder:/FIGURES-TIFF/Description: Raw TIFF images of SDS–PAGE gels.File:Montanha-et-al_RawData_Fig-5_SDS_Page.csvDescription: Densitometric analysis of SDS–PAGE protein bands.------------------------------------------------------------------------------------------------------------------------------------------------/Figure_6Data for 2D FTIR chemical imagingFolder:/FULL_MAPS/Description: 2D FTIR maps corresponding to integrated spectral bands for:- Amide I (protein content)- Lipids- PectinsFile:Montanha-et-al_RawData_Fig-6_FTIR-hotspots.csvDescription: Integrated spectral band values recorded at selected hotspots used for quantitative analysis.------------------------------------------------------------------------------------------------------------------------------------------------/Figure_7Data for synchrotron-based XRF imagingFolder:/RAW_MAPS/Description: Fitted raw XRF maps.File:Montanha-et-al_RawData_Fig-7_XRF.csvDescription: Elemental intensities for:- Potassium (K)- Calcium (Ca)- Zinc (Zn)Intensities correspond to K-alpha emission lines recorded at selected hotspots used for quantitative analysis.The unity for the XRF elemental intensities is counts.------------------------------------------------------------------------------------------------------------------------------------------------/Figure_8Data for Microscopy and micro-CT structural analysisFolder:/IMAGES/Description: Light microscopy images obtained for all analysed genotypes.Files:Montanha-et-al_RawData_Fig-8_Light-microscopy-average.csvDescription: Average values for:- Protein storage vacuole (PSV) area- PSV number- Cell numberMontanha-et-al_RawData_Fig-8_Light-microscopy-PSV-area.csvDescription: Raw measurements of protein storage vacuole area.Montanha-et-al_RawData_Fig-8_uCT-volume.csvDescription: Raw measurements of storage protein vacuole volume obtained using micro-computed tomography (micro-CT).------------------------------------------------------------------------------------------------------------------------------------------------UNITS:The units are described in each------------------------------------------------------------------------------------------------------------------------------------------------CONTACT: Dr. Gabriel Sgarbiero Montanha. E-mail: gabriel.montanha@alumni.usp.br

创建时间:
2026-04-01
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