DataSheet1_Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.).docx

Ethnopharmacological relevance:Salvia glutinosa, also known as the glutinous sage, has been used in Romanian folk medicine in the treatment of inflammation, injuries, and mild infections. However, there is no direct scientific evidence to demonstrate these activities.Aim of the Study: The present research was based on evaluating antioxidant, antiproliferative, and α-glucosidase inhibitory activity of S. glutinosa extracts, as well as the in vivo anti-inflammatory activity.Materials and Methods: Infusions and 70% (v:v) ethanol solution extracts of S. glutinosa stems and leaves, collected from two different locations in Romania, were prepared. Ten phenolic compounds were identified and quantified using the LC-DAD-ESI/MSn method, and total phenolic and flavonoid content, as well as in vitro antioxidant (DPPH, ABTS, and FRAP assays), antiproliferative, anti-inflammatory and alpha-glucosidase inhibitory activities were determined. A rat model of induced inflammation with turpentine oil was used for the examination of in vivo effects of the extracts, using diclofenac as an anti-inflammatory control.Results: The highest inhibitory α-glucosidase activity was determined to be IC50 = 0.546 mg/ml for the hydroalcoholic extract made with plant material collected on the road to Sighișoara. The highest cytotoxic activity against HepG2 cell line was determined to be GI50 = 131.68 ± 5.03 μg/ml, for the hydroalcoholic extract made with plant material from Sighișoara. In vivo administration of extract (200 mg lyophilized powder/ml) showed a significant reduction of NO production.Conclusion: Our findings indicate that S. glutinosa extracts exhibit antioxidant, α-glucosidase inhibitory activity, as well as a modest cytotoxic effect on HepG2 cell line. By in vivo administration, the extracts show anti-inflammatory and antioxidant activity, which correlates with the traditional use of the species. The environmental conditions seemed to induce important changes in the chemical composition and the bioactivity of the herbal preparations derived from S. glutinosa.

民族药理学相关性：Salvia glutinosa，亦称粘性鼠尾草，在罗马尼亚民间医学中被用于治疗炎症、创伤和轻度感染。然而，尚无直接科学证据证明其活性。研究目的：本研究旨在评估S. glutinosa提取物中的抗氧化、抗增殖和α-葡萄糖苷酶抑制活性，以及其体内抗炎活性。材料与方法：制备了从罗马尼亚两个不同地点收集的S. glutinosa茎和叶的浸泡液和70%（体积比）乙醇溶液提取物。利用LC-DAD-ESI/MSn方法鉴定并定量了十种酚类化合物，并测定了总酚和黄酮含量，以及体外抗氧化（DPPH、ABTS和FRAP试验）、抗增殖、抗炎和α-葡萄糖苷酶抑制活性。使用松节油诱导的炎症大鼠模型，以双氯芬酸作为抗炎对照，考察了提取物在体内的作用。结果：对从锡吉什奥拉道路收集的植物材料制成的乙醇提取物，其最高α-葡萄糖苷酶抑制活性被确定为IC50 = 0.546 mg/ml。对从锡吉什奥拉采集的植物材料制成的乙醇提取物，其对HepG2细胞系的最高细胞毒性活性被确定为GI50 = 131.68 ± 5.03 μg/ml。体内给药提取物（200 mg冻干粉末/ml）显示出NO产生量的显著减少。结论：我们的研究结果表明，S. glutinosa提取物表现出抗氧化、α-葡萄糖苷酶抑制活性，以及对HepG2细胞系具有适度的细胞毒性。通过体内给药，提取物显示出抗炎和抗氧化活性，这与该物种的传统使用相符合。环境条件似乎诱导了从S. glutinosa制备的草药制剂的化学成分和生物活性的重要变化。