Stem Cell transcriptional response to Microglia-Conditioned Media. Stem Cell transcriptional response to Microglia-Conditioned Media

This paper demonstrates that whereas SOX2+ cells in HDB are highly susceptible to ZKV, SOX2+ primary GBM samples exhibit moderate to high resistance to infection. Expression profiling revealed an innate immune signature due to microglia infiltration in highly resistant GBM samples that was lacking in HDB. This data was generated to proflile the transcriptional response to microglia-secreted factors in stem cells from glioma cell lines and human developing brain. We find that Interferon-stimulated genes are the main targets upregulated in response to microglia conditioned media exposure. Overall design: GCGR glioma stem cell lines (G322, G334) and primary human developing brain cultures (BRC2251) were exposed to microglia-conditioned media (067,069,070) or to GSC-conditioned control media (G322, G334, BRC2251) media