PTEN intact and NULL prostates in intact and castrate mice

We performed expression profiling of prostates of 3 month wild-type and PTEN NULL mice and assessed the response to 3 days of castration. Seven three month old WT and PbCre x PTEN f/f (PTEN NULL) mice were used. They are in the C57B6 background. Three mice in each group were castrated. Three days after castration, the prostate were harvested and RNA isolated by standard protocols and analyzed by expression profiling.本研究对3月龄野生型（wild-type）与PTEN敲除（PTEN NULL）小鼠的前列腺组织开展表达谱分析，并评估其对3天去势处理的应答反应。本实验共纳入7只3月龄小鼠，涵盖野生型（WT）及PbCre x PTEN f/f（PTEN NULL）两种基因型，所有受试小鼠均为C57B6背景品系。每组各3只小鼠接受去势处理，去势3天后采集前列腺组织，依照标准实验流程提取总RNA并进行表达谱分析。