OMAP-34: Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Esophagus with CODEX

OMAP-34 was designed for CODEX imaging of human fresh frozen esophagus samples (https://doi.org/10.1016/j.cell.2018.07.010; https://doi.org/10.1038/s41596-021-00556-8). The panel contains 31 antibodies; β-Catenin (CTNNB1) was used for cell segmentation while Hoechst served as the nuclear marker. This OMAP provides a spatial context for 8 anatomical structures present in the esophagus: basal epithelium, suprabasal epithelium, superficial epithelium, lamina propria, muscularis mucosae, submucosa, muscularis propria, and blood vessels. Within these layers, the panel covers 18 cell types, including basal, suprabasal, and superficial squamous epithelial cells, stromal fibroblasts, endothelial cells, smooth muscle/myofibroblasts, and multiple immune subsets. Several markers in the panel capture disease-related states such as fibrosis and stromal activation, DNA damage and tumor suppression, and cellular stress or apoptosis. Images were acquired using the Akoya Biosciences Phenocycler Fusion system. For visualization purposes, the minimum and maximum intensity ranges of certain markers were adjusted to enhance signal clarity. All images are provided in .tif format and include corresponding metadata files in .csv format.