RNA-seq of Bacillus amyloliquefaciens

Natural Bacillus isolates generate limited amounts of surfactin (< 10% of their biomass), which functions as an antibiotic or signalling molecule in inter-/intra-specific interactions. However, overproduction hyper-synthesis of surfactin in Bacillus amyloliquefaciens MT45 was observed at a titre of 2.93 g/l, which is equivalent to half of the maximum biomass. The genome and transcriptome of this bacterium were compared with those of B. amyloliquefaciens type strain DSM7T to systemically unravel this efficient biosynthetic mechanism,