Competent B. subtilis cells auxotrophic for methionine were transformed with chromosomal DNA from a met+ (SB19) strain. The yield of met+ transformants (chromosomal transformation), kanamycin-resistan
The bacteria were transformed with equal molar amounts of each of the plasmid DNA. Statistical analysis of the data was performed adopting one way ANOVA analysis (using GraphPad Prism version 5.01) an