James R. LaFountain, Rudolf Oldenbourg (2011) CIL:12372, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Time series images showing the dynamics of kinetchore microtubules during meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that were experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 15 sec using a QImaging Retigo EXi CCD camera. Raw images were processed
using 5-frame algorithm (Shribak and Oldenbourg, 2003). A movie of the time series is included in this grouped set.

本数据集展示了家蝇Nephrotoma suturalis初级精母细胞在有丝分裂I期中动粒体微管动态变化的时间序列图像。采用尼康Microphot SA显微镜进行时间延迟偏振显微镜观察，该显微镜配备液晶偏振光显微镜系统（LC-PolScope，CRi，马萨诸塞州Woburn），配以60倍/1.4 PlanApo油浸物镜，1.4 NA油浸聚光镜，并配备2.0倍变焦镜头。图像每15秒由QImaging Retigo EXi CCD相机捕捉。原始图像采用5帧算法（Shribak和Oldenbourg，2003年）进行处理。本组数据集包含了一系列时间序列图像的动画。